Mfg Part Number
Ab01311-2.0
Vector Labs Ab01311-2.0 Anti-DMC1 [2H12/4], Mouse IgG2a, kappa (200 μg)
Quick Overview
This antibody was raised against human DMC1 protein (expressed originally as a 6xHis fusion in E. coli).
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 2H12/4 |
| Synonyms: | Meiotic recombination protein DMC1/LIM15 homolog; DMC1H; LIM15; MGC150473; MGC150472; HsLim15; DMC1 homologue; disrupted meiotic cDNA1; hDMC1 |
| Amount: | 200 μg |
| Application Codes Clone: | IF; IHC; WB; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for DMC1 and does not cross-react with the related protein Rad51. DMC1 is a RecA/RAD51 homologue which is specific for meiosis and critical for meiotic recombination in Eukaryota. |
| Application Notes: | IF: 2H12/4 anti-DMC1 antibody was used to visualise DMC1 on chromatin in I-L2-NS endopolyploid tumour cells in experiments investigating the role of another protein - REC8 in chromosome segregation after radiation (Erenpreisa et al., 2009). This antibody was also utilised to stain paraffin-embedded cervical cancer tissue samples against DMC1 (Ianzini et al., 2009). IF: Hopkins et al. (2014) successfully used this antibody to stain chromatin spreads from purified testicular germ cells of Stag3+/− and Stag3−/− mice for DMC1 in the studies on the role of Stag3. IF: Another group also used this antibody for the immunofluorescence staining of spermatocyte chromosome spreads against DMC1 in a study analysing proteins involved in telomeres' attachment to the nuclear envelope (Wang et al., 2019). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |