Mfg Part Number
Ab01312-1.6

Vector Labs Ab01312-1.6 Anti-Rad51 [3C10], Mouse Fab fragment, His-Tagged, kappa (100 μg)

Quick Overview
This antibody was raised against the recombinant human RAD51 protein.
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Product Class:Purified
Clone ID:3C10
Heavy Chain Modification:His-Tagged
Synonyms:DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A; BRCC5; HsT16930; RAD51 homolog; homolog of RecA, E. coli; RecA-like protein; RAD51ABRCA1/BRCA2-containing complex, subunit 5; Ab1; Ab-1; Rad51 Ab1; 51RAD01
Amount:100 μg
Application Codes Clone:WB; IF; IP; IHC
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody recognises RAD51 (and might also recognise hDmc1 (Masson et al., 1999)). RAD51 is a eukaryotic homologue of E. coli RecA. It is an important part of the homologous recombination DNA repair pathway during the homologous strand exchange stage.
Application Notes:WB: Anti-Rad51 3C10 antibody was used to visualise Rad51 via Western blot after previous immunoprecipitation with different antibodies in experiments analysing the interactions between Rad51 and DMC1 (Masson et al., 1999). IF: 3C10 antibody was used to visualise Rad51 on chromatin in endopolyploid tumour cells in experiments investigating the role of another protein - REC8 in chromosome segregation after radiation (Erenpreisa et al., 2009). IHC: This antibody was also used to immunohistochemically stain biopsy and resected specimens of esophagus and squamous cell carcinoma of the esophagus (Nakanoko et al., 2014).
More Information
Brand Vector Labs
UNSPSC 12161500