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Mfg Part Number

Ab01315-10.0

Vector Labs Ab01315-10.0 Anti-CD22 [To15], Human IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antobody was raised by immunising Balb-c mice with a potassium iodide extract of normal human tonsil cell membranes and subsequent creation of hybridomas.

$517.40

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Description

Product Class: Purified
Clone ID: To15
Synonyms: Siglec2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; SIGLEC2FLJ22814
Amount: 200 μg
Application Codes Clone: IHC; FACS; IP; adhesion assays
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognises epitope C on the fourth Ig-like domain of the human CD22. CD22 which is a representative of the immunoglobulin superfamiliy and binds sialylated ligands. It's involved in the interactions between B-cell interactions. It is higly expressed in leukaemias and many other B cell neoplasms.
Application Notes: IP: To15 antibody was used for the immunoprecipitation of the products of IgM kinase reaction to examine association between CD22 and B-cell receptor (Peaker and Neurberger, 1993); another group was also successfull in using this abtybody for immunoprecipitation (Zhang and Varki, 2004). Furthermore, it was demonstrated that To15 antibody was able to block CD22-mediated adhesion (specifically, the binding of Daudi cells to COS-CD cells) in around 40% of cases, which presents a potential for using this antibody in CD22 inhibition in the immune disease (Tedder et al., 1996). IHC: This antibody was also used to co-stain histological samples of lymph node biopsies in an attempt to immunologically characterise Langerhans cells in lymph nodes (Ralfkiaer et al., 1984).

Product Class:	Purified
Clone ID:	To15
Synonyms:	Siglec2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; SIGLEC2FLJ22814
Amount:	200 μg
Application Codes Clone:	IHC; FACS; IP; adhesion assays
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognises epitope C on the fourth Ig-like domain of the human CD22. CD22 which is a representative of the immunoglobulin superfamiliy and binds sialylated ligands. It's involved in the interactions between B-cell interactions. It is higly expressed in leukaemias and many other B cell neoplasms.
Application Notes:	IP: To15 antibody was used for the immunoprecipitation of the products of IgM kinase reaction to examine association between CD22 and B-cell receptor (Peaker and Neurberger, 1993); another group was also successfull in using this abtybody for immunoprecipitation (Zhang and Varki, 2004). Furthermore, it was demonstrated that To15 antibody was able to block CD22-mediated adhesion (specifically, the binding of Daudi cells to COS-CD cells) in around 40% of cases, which presents a potential for using this antibody in CD22 inhibition in the immune disease (Tedder et al., 1996). IHC: This antibody was also used to co-stain histological samples of lymph node biopsies in an attempt to immunologically characterise Langerhans cells in lymph nodes (Ralfkiaer et al., 1984).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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