Mfg Part Number
Ab01346-1.1
Vector Labs Ab01346-1.1 Anti-Biotin [BK-1/39], Mouse IgG1, kappa (200 μg)
Quick Overview
This antibody was raised by immunising mice with biotin conjugated to keyhole limpet haemocyanin.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | BK-1/39 |
| Synonyms: | vitamin B7; vitamin H; coenzyme R |
| Amount: | 200 μg |
| Application Codes Clone: | IHC; FC; IP; ChIP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognises biotin (vitamin B7). It is a water-soluble vitamin which serves as a cofactor of important metabolic enzymes involved in processing of carbohydrates, lipids and amino acids as well as carbon dioxide transport. |
| Application Notes: | This monoclonal antibody can be used to detect biotinylated proteins and antibodies, particularly if signal amplification is desired. For instance, PE-conjugated BK-1/39 antibody was used to target biotinylated anti-Ig beta antibody in a flow cytometric analysis (Levit-Zerdoun et al., 2016). This antibody was also successfully utilised in the chromatin immunoprecipitation (ChIP) of biotinylated sense or antisense strands of the saRNA duplex (Wu et al., 2016). BK-1/39 was used in in situ hybridisation as well; it detected biotinylated RNA probe complementary to the Epstein-Barr virus genome (de Lima et al., 2012). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |