Mfg Part Number
Ab01354-10.3
Vector Labs Ab01354-10.3 Anti-Do (b) [Mima-73], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunising BALB/c mice with human embryonic kidney 293T cells transfected to express high levels of Dombrock b.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | Mima-73 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CD297; Dob; Dombrock glycoprotein; Dombrock (b); 15D6; ADP-ribosyltransferase 4; Ecto-ADP-ribosyltransferase 4; ART4; ARTC4; DO; DO/ART4; DOK1 |
| Amount: | 200 μg |
| Application Codes Clone: | FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2c |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2c format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for Dombrock b, a blood group antigen carried by ART4. Haemagglutination studies have shown this antibody to react with RBCs from non-human primates, including chimpanzee and orangutan (Mogos et al, 2003). |
| Application Notes: | The specificity of this antibody has been confirmed in haemagglutination studies, where it reacts with Do(a-b+) and Do(a+b+) RBCs, but not with Do(a+b-) RBCs (Grodecka et al, 2012). The epitope recognised by this antibody is resistant to treatment with papain, alpha-chymotrypsin and neuraminidase, and sensitive to treatment with trypsin and DTT (Grodecka et al, 2012). This antibody has been used, as part of a panel of antibodies specific for different epitopes on the Dombrock glycoprotein, in flow cytometric and haemagglutination analysis of human red blood cells (Mayer et al, 2010; Costa et al, 2010). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |