Mfg Part Number
Ab01355-10.3

Vector Labs Ab01355-10.3 Anti-Do (b) [Mima-143], Human IgG1, Fc Silent™, kappa (200 μg)

Quick Overview
This antibody was raised by immunising BALB/c mice with human embryonic kidney 293T cells transfected to express high levels of Dombrock b.
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Product Class:Purified
Clone ID:Mima-143
Heavy Chain Modification:Fc Silent™
Synonyms:CD297;Dob; Dombrock glycoprotein; Dombrock (b); 1G8; ADP-ribosyltransferase 4; Ecto-ADP-ribosyltransferase 4; ART4; ARTC4; DO; DO/ART4; DOK1
Amount:200 μg
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2c
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2c format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for Dombrock b, a blood group antigen carried by ART4.
Application Notes:The specificity of this antibody has been confirmed in haemagglutination studies, where it reacts with Do(a-b+) and Do(a+b+) RBCs, but not with Do(a+b-) RBCs (Grodecka et al, 2012). The epitope recognised by this antibody is resistant to treatment with papain, alpha-chymotrypsin and neuraminidase, and sensitive to treatment with trypsin and DTT (Grodecka et al, 2012).
More Information
Brand Vector Labs
UNSPSC 12161500