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Mfg Part Number

Ab01369-10.3

Vector Labs Ab01369-10.3 Anti-CEACAM1 [B3-17], Human IgG1, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising mice with recombinant human CEACAM1.

$517.40

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Description

Product Class: Purified
Clone ID: B3-17
Heavy Chain Modification: Fc Silent™
Synonyms: CD66a; Carcinoembryonic antigen-related cell adhesion molecule 1; Biliary glycoprotein 1; BGP-1; BGPI
Amount: 200 μg
Application Codes Clone: IP; IHC; IF; FC; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human CEACAM1, and recognises an epitope in the extracellular domain.
Application Notes: In flow cytometric analysis, this antibody has been used to detect CEACAM1 surface expression by human epithelial and endothelial cell lines, and microvesicles derived from these cells (Muturi et al, 2013), normal human bronchial epithelial (NHBE) cells (Klaile et al, 2013), CEACAM1-knockout human intestinal epithelial cells (Klaile et al, 2017) and human B-cell subpopulations (Khairnar et al, 2015; Seifert et al, 2015). It has also been used to detect CEACAM1 expression in ELISA analysis of transfected A549 epithelial cells (Singer et al, 2014). This antibody has been used in immunofluorescence analysis of NHBE cells (Klaile et al, 2013), as well as in the immunoprecipitation of CEACAM1 from NHBE cell culture supernatants (Klaile et al, 2013). Additionally, this antibody has been used in IHC staining of paraffin-embedded human lung tissue sections (Klaile et al, 2013).

Product Class:	Purified
Clone ID:	B3-17
Heavy Chain Modification:	Fc Silent™
Synonyms:	CD66a; Carcinoembryonic antigen-related cell adhesion molecule 1; Biliary glycoprotein 1; BGP-1; BGPI
Amount:	200 μg
Application Codes Clone:	IP; IHC; IF; FC; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human CEACAM1, and recognises an epitope in the extracellular domain.
Application Notes:	In flow cytometric analysis, this antibody has been used to detect CEACAM1 surface expression by human epithelial and endothelial cell lines, and microvesicles derived from these cells (Muturi et al, 2013), normal human bronchial epithelial (NHBE) cells (Klaile et al, 2013), CEACAM1-knockout human intestinal epithelial cells (Klaile et al, 2017) and human B-cell subpopulations (Khairnar et al, 2015; Seifert et al, 2015). It has also been used to detect CEACAM1 expression in ELISA analysis of transfected A549 epithelial cells (Singer et al, 2014). This antibody has been used in immunofluorescence analysis of NHBE cells (Klaile et al, 2013), as well as in the immunoprecipitation of CEACAM1 from NHBE cell culture supernatants (Klaile et al, 2013). Additionally, this antibody has been used in IHC staining of paraffin-embedded human lung tissue sections (Klaile et al, 2013).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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