Vector Labs Ab01375-13.12 Anti-MS4A4A [3F2], Human IgG4-S228P, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 3F2
Synonyms: Four-span transmembrane protein 1; human membrane-spanning 4-domains subfamily A member 4A; CD20 antigen-like 1; (MS4A4A)-GFP; MS4A4; 4SPAN1; MS4A
Amount: 200 μg
Application Codes Clone: FC; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human extracellular MS4A4A. The 3F2 and 4H2 (Ab01373) mAb clones completely cross-block one another indicating that they might bind to the same epitope or proximal epitopes (Sanyal et al., 2017; pmid: 28303902). MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. MS4A4A is likely to be a component of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.
Application Notes: 3F2 is recommended for the detection and analysis of expression of MS4A4A. Importantly, MS4A4A has been shown to be a marker for M2 macrophages and plasma cells and to be overexpressed in multiple myelomas, suggesting a potential role in targeting alternatively activated macrophages such as tumor-associated macrophages, and in the treatment of multiple myeloma and mantle cell lymphoma (Sanyal et al., 2017; pmid: 28303902). The antibody 3F2 can be successfully used for the detection of MS4A4A-expressing cells via flow cytometry (Sanyal et al., 2017; pmid: 28303902). Finally, specificity of this antibody was additionally confirmed via immunoprecipitation of lysates of BJAB MS4A4A-HA cells (Sanyal et al., 2017; pmid: 28303902).

Product Class:	Purified
Clone ID:	3F2
Synonyms:	Four-span transmembrane protein 1; human membrane-spanning 4-domains subfamily A member 4A; CD20 antigen-like 1; (MS4A4A)-GFP; MS4A4; 4SPAN1; MS4A
Amount:	200 μg
Application Codes Clone:	FC; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human extracellular MS4A4A. The 3F2 and 4H2 (Ab01373) mAb clones completely cross-block one another indicating that they might bind to the same epitope or proximal epitopes (Sanyal et al., 2017; pmid: 28303902). MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. MS4A4A is likely to be a component of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.
Application Notes:	3F2 is recommended for the detection and analysis of expression of MS4A4A. Importantly, MS4A4A has been shown to be a marker for M2 macrophages and plasma cells and to be overexpressed in multiple myelomas, suggesting a potential role in targeting alternatively activated macrophages such as tumor-associated macrophages, and in the treatment of multiple myeloma and mantle cell lymphoma (Sanyal et al., 2017; pmid: 28303902). The antibody 3F2 can be successfully used for the detection of MS4A4A-expressing cells via flow cytometry (Sanyal et al., 2017; pmid: 28303902). Finally, specificity of this antibody was additionally confirmed via immunoprecipitation of lysates of BJAB MS4A4A-HA cells (Sanyal et al., 2017; pmid: 28303902).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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