Mfg Part Number
Ab01384-23.0
Vector Labs Ab01384-23.0 Anti-MARCO [PLK1], Rabbit IgG, kappa (200 μg)
Quick Overview
The antibody was raised by immunising BALB/c mice with human alveolar macrophages (AMs) and subsequent creation of hybridomas.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | PLK1 |
| Synonyms: | SCARA2; macrophage receptor MARCO; macrophage receptor with collagenous structure; scavenger receptor class A member 2; PLK-1; PLK 1 |
| Amount: | 200 μg |
| Application Codes Clone: | IF; IHC-Fr; IP; WB; flow cytometry |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG3 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises the extracellular domain V between residues 420 and 431 in MARCO both in human and bovine samples. |
| Application Notes: | The specificity of this antibody was confirmed in an IF experiment where the antibody stained alveolar macrophages for MACRO (Arredouani et al., 2005). Tissue samples prepared in acetone and paraformaldehyde were also successfully immunohistochemically stained (Arredouani et al., 2005; Martens et al., 2006). The antibody was also capable of weak staining of alveolar macrophages in flow cytometry (Palecanda and Kobzik, 2001). In IP experiments, the antibody immunoprecipitates MARCO from lysates obtained from COS cells transfected with human MARCO (Arredouani et al., 2005). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |