Mfg Part Number
Ab01407-23.0
Vector Labs Ab01407-23.0 Anti-Asp f 1 [14H4], Rabbit IgG, lambda (200 μg)
Quick Overview
14H4 antibody was raised using a novel technique: mononuclear cells were taken from the human subjects with allergic bronchopulmonary aspergillosis and then screened for IgE secretion. Subsequently, they were used to generate hybridomas of which some were secreting anti-Asp f 1 IgE.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 14H4 |
| Synonyms: | A. fumigatus Allergen; Asp f1; Aspf1; ribotoxin Asp f 1; ribonuclease mitogillin; allergen Asp f I; Asp f I; IgE-binding ribotoxin; major allergen Asp f 1; allergen: Asp f 1 |
| Amount: | 200 μg |
| Application Codes Clone: | WB; ELISA; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgE |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgE format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises Asp f 1. |
| Application Notes: | This antibody's target protein (Asp f 1) was confirmed through IP and mass spectrometry analysis (Wurth et al., 2018). WB and ELISA: the antibody was successfull in detecting Asp f 1 antigen in a commercial Aspergillus extract ALKB (Wurth et al., 2018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |