Mfg Part Number
Ab01408-20.0
Vector Labs Ab01408-20.0 Anti-Asp f9 [5C6], Mouse IgE, kappa (50 μg)
Quick Overview
5C6 antibody was raised using a novel technique: mononuclear cells were taken from the human subjects with allergic bronchopulmonary aspergillosis and then screened for IgE secretion. Subsequently, they were used to generate hybridomas of which some were secreting anti-Asp f 9 IgE.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 5C6 |
| Synonyms: | A. fumigatus Allergen; Asp f 9; Aspf9; probable glycosidase crf1; Crh-like protein 1; glucanase; allergen: Asp f 9; allergen Asp f 9 |
| Amount: | 50 μg |
| Application Codes Clone: | WB; ELISA; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgE |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Human IgE format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises Asp f 9. |
| Application Notes: | A target of the 5C6 antibody (Asp f 9) was confirmed through IP and mass spectrometry (Wurth et al., 2018). ELISA: this antibody detected Asp f 9 in a commercial Aspergillus extract ALKB in ELISA (Wurth et al., 2018). WB: 5C6 antibody was successful in detecting Asp f 9 in western blot samples from Aspergillus extracts HS and AlgryL (Wurth et al., 2018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |