Mfg Part Number
Ab01414-1.1
Vector Labs Ab01414-1.1 Anti-metarhodopsin-I [K42-41L], Mouse IgG1, kappa (200 μg)
Quick Overview
This antibody was raised by immunising mice with rhodopsin.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | K42-41L |
| Synonyms: | opsin-2; meta I rhodopsin; rhodopsin; opsin 2; metarhodopsin I |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; antibody imprint; EM ICC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognises secifically C3 loop of metarhodopsin-I (residues 230-252) (and also dark-adapted form of rhodopsin) but it does not bind to the ligh-activated form - metarhodopsin-II. Rhodopsin is the best studied GPCR and is present in rod cells where it is activated by light being responsible for the signal transduction in the visual system. |
| Application Notes: | Antibody imprint: This antibody was used to investigate the structure of rhodopsin's meta-states; the researchers employed the antibody imprint technique to analyse conformational changes which this molecule undergoes upon activation by light (Bailey et al., 2003). Another group used K42-41L antibody to demonstrate that it is capable of discriminating between metarhodopsins I and II, and that it stabilises metarhodopsin I while inhibiting metarhodopsin II; these data suggest that this antibody might be useful in the structural studies of opsins (Piscitelli et al., 2006). EM ICC: K42-41L antibody was utilised to stain different structures of Xenopus laevis retinas in EM immunocytochemistry LR white samples in a study investigating opsin localisation (Besharse and Wetzel, 1995). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |