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Mfg Part Number

Ab01414-23.0

Vector Labs Ab01414-23.0 Anti-metarhodopsin-I [K42-41L], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising mice with rhodopsin.

$517.40

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Description

Product Class: Purified
Clone ID: K42-41L
Synonyms: opsin-2; meta I rhodopsin; rhodopsin; opsin 2; metarhodopsin I
Amount: 200 μg
Application Codes Clone: ELISA; antibody imprint; EM ICC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognises secifically C3 loop of metarhodopsin-I (residues 230-252) (and also dark-adapted form of rhodopsin) but it does not bind to the ligh-activated form - metarhodopsin-II. Rhodopsin is the best studied GPCR and is present in rod cells where it is activated by light being responsible for the signal transduction in the visual system.
Application Notes: Antibody imprint: This antibody was used to investigate the structure of rhodopsin's meta-states; the researchers employed the antibody imprint technique to analyse conformational changes which this molecule undergoes upon activation by light (Bailey et al., 2003). Another group used K42-41L antibody to demonstrate that it is capable of discriminating between metarhodopsins I and II, and that it stabilises metarhodopsin I while inhibiting metarhodopsin II; these data suggest that this antibody might be useful in the structural studies of opsins (Piscitelli et al., 2006). EM ICC: K42-41L antibody was utilised to stain different structures of Xenopus laevis retinas in EM immunocytochemistry LR white samples in a study investigating opsin localisation (Besharse and Wetzel, 1995).

Product Class:	Purified
Clone ID:	K42-41L
Synonyms:	opsin-2; meta I rhodopsin; rhodopsin; opsin 2; metarhodopsin I
Amount:	200 μg
Application Codes Clone:	ELISA; antibody imprint; EM ICC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognises secifically C3 loop of metarhodopsin-I (residues 230-252) (and also dark-adapted form of rhodopsin) but it does not bind to the ligh-activated form - metarhodopsin-II. Rhodopsin is the best studied GPCR and is present in rod cells where it is activated by light being responsible for the signal transduction in the visual system.
Application Notes:	Antibody imprint: This antibody was used to investigate the structure of rhodopsin's meta-states; the researchers employed the antibody imprint technique to analyse conformational changes which this molecule undergoes upon activation by light (Bailey et al., 2003). Another group used K42-41L antibody to demonstrate that it is capable of discriminating between metarhodopsins I and II, and that it stabilises metarhodopsin I while inhibiting metarhodopsin II; these data suggest that this antibody might be useful in the structural studies of opsins (Piscitelli et al., 2006). EM ICC: K42-41L antibody was utilised to stain different structures of Xenopus laevis retinas in EM immunocytochemistry LR white samples in a study investigating opsin localisation (Besharse and Wetzel, 1995).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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