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Mfg Part Number

Ab01416-1.6

Vector Labs Ab01416-1.6 Anti-digoxin [1B3], Mouse Fab fragment, His-Tagged, kappa (100 μg)

VECTOR LABS

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Quick Overview

This antibody was raised using transgenic HC2/KCo5 mice with the humanised humoral immune system capable of generating human IgG1 κ Abs.The mice were immunised with digoxin conjugated to keyhole limpet hemocyanin (KLH).

$692.90

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Description

Product Class: Purified
Clone ID: 1B3
Heavy Chain Modification: His-Tagged
Synonyms: 12β-hydroxydigitoxin; digitalis; 12-beta-hydroxydigitoxin
Amount: 100 μg
Application Codes Clone: depletion; therapy
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: 1B3 antibody recognises digoxin and digitoxin with an excellent binding affinity of Kd = 3 and 0.6 nM, respectively. Digoxin and digitoxin are both cardiac glycosides used widely in cardiology to treat various heart conditions, such as heart failure.
Application Notes: Digoxin is widely used in various heart conditions. However, the drug has a very narrow therapeutic index and can cause toxic side effects. The antibody might neutralise the activity of digoxin and could be used as an antidote in case of overdose of digitalis (Paula et al., 2005). It is additionally superior to the widely utilised Fab preparations of sheep polyclonal anti-digoxin Abs as it is a humanised antibody which will not activate the immune system in patients (Ball et al., 1999). Anti-digoxin monoclonal antibodies can also be used to measure serum levels of the drug as a guide to therapy, and are useful in the investigation of the kinetics of interaction between antibody and antigen.

Product Class:	Purified
Clone ID:	1B3
Heavy Chain Modification:	His-Tagged
Synonyms:	12β-hydroxydigitoxin; digitalis; 12-beta-hydroxydigitoxin
Amount:	100 μg
Application Codes Clone:	depletion; therapy
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	1B3 antibody recognises digoxin and digitoxin with an excellent binding affinity of Kd = 3 and 0.6 nM, respectively. Digoxin and digitoxin are both cardiac glycosides used widely in cardiology to treat various heart conditions, such as heart failure.
Application Notes:	Digoxin is widely used in various heart conditions. However, the drug has a very narrow therapeutic index and can cause toxic side effects. The antibody might neutralise the activity of digoxin and could be used as an antidote in case of overdose of digitalis (Paula et al., 2005). It is additionally superior to the widely utilised Fab preparations of sheep polyclonal anti-digoxin Abs as it is a humanised antibody which will not activate the immune system in patients (Ball et al., 1999). Anti-digoxin monoclonal antibodies can also be used to measure serum levels of the drug as a guide to therapy, and are useful in the investigation of the kinetics of interaction between antibody and antigen.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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