Mfg Part Number
Ab01419-1.6
Vector Labs Ab01419-1.6 Anti-PD-L1 [10F.9G2], Mouse Fab fragment, His-Tagged, kappa (100 μg)
Quick Overview
This antibody was raised by immunising Lewis rats with murine PD-L1 cDNA and murine PD-L1 CHO transfectants.
$692.90
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| Product Class: | Purified |
|---|---|
| Clone ID: | 10F.9G2 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | CD274; PDL1; PD L1; Programmed cell death 1 ligand 1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; 10F9G2; 10F 9G2 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; FC; IHC; blocking |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for murine PD-L1. |
| Application Notes: | The specificity of this antibody has been confirmed in ELISA analysis (Eppihimer et al, 2002). This antibody reacts specifically with mPD-L1-transfected cells in flow cytometry, and has been used to assess microvascular endothelial cells PD-L1 expression (Eppihimer et al, 2002). When administered to mice, a radiolabelled version of this antibody has been used to quantify PD-L1 expression in vivo, by measuring antibody accumulation in different tissues (Eppihimer et al, 2002). This antibody has also been used in immunohistochemical analysis of PD-L1 expression in frozen tissue sections of murine brain (Eppihimer et al, 2002) and murine hearts (Rodig et al, 2003). This antibody has been shown to block the binding of PD-L1 to both PD-1 and B7-1 (Paterson et al, 2011). This antibody precipitates diabetes in NOD mice and in adoptive transfer models of CD4+ and CD8+ T cell-driven diabetes, and accelerates diabetes in recipients of T cells from diabetic and prediabetic mice (Paterson et al, 2011). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |