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Mfg Part Number

Ab01420-1.32

Vector Labs Ab01420-1.32 Anti-VISTA [13F3], Mouse IgG1-D265A, Fc Silenced, lambda (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising Armenian hamsters with EL4 cells overexpressing VISTA-RFP, and VISTA-Ig fusion protein.

$517.40

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Description

Product Class: Purified
Clone ID: 13F3
Heavy Chain Modification: Fc Silenced
Synonyms: PD-1H; V-domain Ig suppressor of T cell activation; V-type immunoglobulin domain-containing suppressor of T-cell activation; Platelet receptor Gi24; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; Vsir; Dies1
Amount: 200 μg
Application Codes Clone: ELISA; FC; blocking
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for murine VISTA.
Application Notes: The specificity of this antibody has been confirmed in both ELISA and flow cytometry (Wang et al, 2011). This antibody has been used in flow cytometric analysis to assess VISTA expression in murine lupus models (Ceeraz et al, 2017; Sergent et al, 2018) and mice infected with HSV-1 (Srivastava et al, 2018). This antibody has been shown to block VISTA, and enhances T cell proliferation in vitro (Wang et al, 2011). In mice models, this antibody exacerbates experimental autoimmune encephalomyelitis (Wang et al, 2011) and murine arthritis and lupus disease progression (Sergent et al, 2018). The use of this antibody to achieve a VISTA blockade enhances anti-tumour immune responses in mice models of both transplantable and inducible melanoma, suppressing tumour growth (LeMercier et al, 2014).

Product Class:	Purified
Clone ID:	13F3
Heavy Chain Modification:	Fc Silenced
Synonyms:	PD-1H; V-domain Ig suppressor of T cell activation; V-type immunoglobulin domain-containing suppressor of T-cell activation; Platelet receptor Gi24; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; Vsir; Dies1
Amount:	200 μg
Application Codes Clone:	ELISA; FC; blocking
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for murine VISTA.
Application Notes:	The specificity of this antibody has been confirmed in both ELISA and flow cytometry (Wang et al, 2011). This antibody has been used in flow cytometric analysis to assess VISTA expression in murine lupus models (Ceeraz et al, 2017; Sergent et al, 2018) and mice infected with HSV-1 (Srivastava et al, 2018). This antibody has been shown to block VISTA, and enhances T cell proliferation in vitro (Wang et al, 2011). In mice models, this antibody exacerbates experimental autoimmune encephalomyelitis (Wang et al, 2011) and murine arthritis and lupus disease progression (Sergent et al, 2018). The use of this antibody to achieve a VISTA blockade enhances anti-tumour immune responses in mice models of both transplantable and inducible melanoma, suppressing tumour growth (LeMercier et al, 2014).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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