Mfg Part Number
Ab01428-23.0
Vector Labs Ab01428-23.0 Anti-CD53 [HD77], Rabbit IgG, kappa (200 μg)
Quick Overview
This antibody was raised by immunising mice with human leukemia cells.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | HD77 |
| Synonyms: | TSPAN-25; Leukocyte surface antigen CD53; Tetraspanin-25; MOX44; TSPAN25 |
| Amount: | 200 μg |
| Application Codes Clone: | FC; IHC; IF; WB; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen. |
| Application Notes: | This antibody has applications in the detection of normal and neoplastic white blood cells. This antibody has been used to stain tumour and mononuclear cells for CD53 expression in flow cytometric analysis of samples from ovarian carcinoma patients (Marme et al, 2002), and cells of the colon carcinoma line HT29 (Koretz et al, 1992). In immunohistochemical analysis of frozen tissue sections, this antibody has been shown to react with osteoclasts and osteoclast-like giant cells in malignant fibrous histiocytoma and in benign giant cell tumor of tendon sheath (Mechtersheimer et al, 1990). This antibody has been used to re-immunoprecipitate CD53 from PKCα immunoprecipiates (Zhang et al, 2001). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |