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Mfg Part Number

Ab01433-23.9

Vector Labs Ab01433-23.9 Anti-Fab (kappa) [TP1170], Rabbit IgG-Fc fusion, His-Tagged, (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.

$517.40

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Description

Product Class: Purified
Clone ID: TP1170
Heavy Chain Modification: His-Tagged
Synonyms: antigen-binding fragment kappa light chain
Amount: 200 μg
Application Codes Clone: WB; IF; ICC; dot blot assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognises mouse kappa light chain.
Application Notes: Anti-IgG nanobodies can often perform more efficiently than polyclonal secondary antibodies in various experimental assays. For instance, this nanobody when conjugated with the infrared fluorophore IRDye 800 was more efficient in terms of the detection sensitivity of IgG2a antibodies than the polyclonal secondary antibody via Western blot (Pleiner et al., 2018). TP1170 can also be used as an efficient secondary nanobody in the indirect immunofluorescence. It was used in combination with other nanobodies to detect both mouse IgG1 and IgG2a primary antibodies giving strong, clean signal without any background staining (Pleiner et al., 2018). This nanobody is also highly recommended for superresolution microscopy, as it can place the fluorophore much closer to the antigen which had been bound by the primary antibody than a usual secondary antibody would be able to (Pleiner et al., 2018).

Product Class:	Purified
Clone ID:	TP1170
Heavy Chain Modification:	His-Tagged
Synonyms:	antigen-binding fragment kappa light chain
Amount:	200 μg
Application Codes Clone:	WB; IF; ICC; dot blot assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognises mouse kappa light chain.
Application Notes:	Anti-IgG nanobodies can often perform more efficiently than polyclonal secondary antibodies in various experimental assays. For instance, this nanobody when conjugated with the infrared fluorophore IRDye 800 was more efficient in terms of the detection sensitivity of IgG2a antibodies than the polyclonal secondary antibody via Western blot (Pleiner et al., 2018). TP1170 can also be used as an efficient secondary nanobody in the indirect immunofluorescence. It was used in combination with other nanobodies to detect both mouse IgG1 and IgG2a primary antibodies giving strong, clean signal without any background staining (Pleiner et al., 2018). This nanobody is also highly recommended for superresolution microscopy, as it can place the fluorophore much closer to the antigen which had been bound by the primary antibody than a usual secondary antibody would be able to (Pleiner et al., 2018).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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