Mfg Part Number
Ab01434-34.11
Vector Labs Ab01434-34.11 Anti-Fab (kappa) [TP975], Camelid VHH, His-tagged, (50 μg)
Quick Overview
This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
$692.90
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | TP975 |
| Heavy Chain Modification: | His-tagged |
| Synonyms: | antigen-binding fragment kappa light chain |
| Amount: | 50 μg |
| Application Codes Clone: | dot blot assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognises mouse kappa light chain. |
| Application Notes: | Anti-IgG nanobodies often perform more efficiently than polyclonal secondary antibodies in various experimental assays. Therefore, they are recommended for various experimental procedures. Anti-IgG nanobodies were shown to be more sensitive during Western blots when conjugated with different molecules (HRP, fluorophores etc.) (Pleiner et al., 2018). Furthermore, combination of two distinct nanobodies for immunofluorescence labelling provided exceptionally clean and strong signal, unprecedented among monovalent secondary antibodies (Pleiner et al., 2018). Additionally, using such an antibody allows skipping two-step incubation procedures during immunostaing as the nanobody can be first incubated with a primary antibody and this conjugate might be added to the cells in a one-step procedure (Pleiner et al., 2018). Finally, this anti-kappa nanobody has been recommended for superresolution microscopy, as it can place the fluorophore much closer to the antigen which had been bound by the primary antibody than a usual secondary antibody would be able to (Pleiner et al., 2018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |