Mfg Part Number
Ab01435-34.11
Vector Labs Ab01435-34.11 Anti-Fab (lambda) [TP1014], Camelid VHH, His-tagged, (50 μg)
Quick Overview
This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
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| Product Class: | Purified |
|---|---|
| Clone ID: | TP1014 |
| Heavy Chain Modification: | His-tagged |
| Synonyms: | antigen-binding fragment lambda light chain |
| Amount: | 50 μg |
| Application Codes Clone: | dot blot assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognises lambda light chain. |
| Application Notes: | Anti-IgG nanobodies often perform more efficiently than polyclonal secondary antibodies in various experimental assays. Therefore, they are recommended for various experimental procedures. Anti-IgG nanobodies were shown to be more sensitive during Western blots when conjugated with different molecules (HRP, fluorophores etc.) (Pleiner et al., 2018). Furthermore, combination of two distinct nanobodies for immunofluorescence labelling provided exceptionally clean and strong signal, unprecedented among monovalent secondary antibodies (Pleiner et al., 2018). Additionally, using such an antibody allows skipping two-step incubation procedures during immunostaing as the nanobody can be first incubated with a primary antibody and this conjugate might be added to the cells in a one-step procedure (Pleiner et al., 2018). Finally, such nanobodies have been recommended as very effective for colocalisation studies when more than one fluorophore is needed (Pleiner et al., 2018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |