Mfg Part Number
Ab01446-23.9
Vector Labs Ab01446-23.9 Anti-IgG1 (hinge) [TP885], Rabbit IgG-Fc fusion, His-Tagged, (200 μg)
Quick Overview
This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
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| Product Class: | Purified |
|---|---|
| Clone ID: | TP885 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | immunoglobulin G1 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; dot blot assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises mouse and rat IgG1, with a proposed epitope located at the hinge region. |
| Application Notes: | TP885 nanobody can be used for the purification of antigens or antigen complexes previously bound to a primary antibody, as it successfully isolated mouse monoclonal IgG1 from hybridoma cell culture supernatant and then released it under physiological conditions using SUMOStar protease cleavage (Pleiner et al., 2018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |