Mfg Part Number
Ab01447-23.9
Vector Labs Ab01447-23.9 Anti-IgG1 Fab (k+l) [TP886], Rabbit IgG-Fc fusion, His-Tagged, (200 μg)
Quick Overview
This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
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| Product Class: | Purified |
|---|---|
| Clone ID: | TP886 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | immunoglobulin G1 antigen-binding fragment kappa light chain; immunoglobulin G1 antigen-binding fragment lambda light chain; kappa light chain; lambda light chain; immunoglobulin G1 |
| Amount: | 200 μg |
| Application Codes Clone: | IF; ICC; dot blot assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises Fab fragment of mouse IgG1 (both with kappa and lambda light chains). |
| Application Notes: | TP886 nanobody can be used as an efficient high-affinity reagent for immunofluorescence. It was utilised in conjugation with two to three fluorophores in detection of mouse monoclonal IgG1 for the indirect immunostaining of HeLa cells where it performed exceptionally well as for a non-polyclonal antibody reaching similar signal strength to polyclonal agents when it was combined with another anti-IgG1 nanobody (Pleiner et al., 2018). Monovalent nanobodies can be also effectively used in a single step immunostaining procedure because they do not create large, cross-linked complexes with primary antibodies.TP886 was used as a secondary reagent; it was first incubated with a primary antibody and then a one-step immunostaining was performed (without the usual separate incubation with a primary and secondary antibody (Pleiner et al., 2018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |