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Mfg Part Number

Ab01449-34.11

Vector Labs Ab01449-34.11 Anti-IgG1/2a Fab (k) [TP888], Camelid VHH, His-tagged, (50 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.

$692.90

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Description

Product Class: Purified
Clone ID: TP888
Heavy Chain Modification: His-tagged
Synonyms: immunoglobulin G1; immunoglobulin G2a; antigen binding fragment; kappa light chain
Amount: 50 μg
Application Codes Clone: dot blot assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody recognises mouse Fab of the IgG1 and IgG2a which contain kappa light chain (the binding seems to be dependent on the CH1 region and the light chain type simultaneously).
Application Notes: Anti-IgG nanobodies often perform more efficiently than polyclonal secondary antibodies in various experimental assays. Therefore, they are recommended for various experimental procedures. Anti-IgG nanobodies were shown to be more sensitive during Western blots when conjugated with different molecules (HRP, fluorophores etc.) (Pleiner et al., 2018). Furthermore, combination of two distinct nanobodies for immunofluorescence labelling provided exceptionally clean and strong signal, unprecedented among monovalent secondary antibodies (Pleiner et al., 2018). Additionally, using such an antibody allows skipping two-step incubation procedures during immunostaing as the nanobody can be first incubated with a primary antibody and this conjugate might be added to the cells in a one-step procedure (Pleiner et al., 2018). Finally, such nanobodies have been recommended as very effective for colocalisation studies when more than one fluorophore is needed (Pleiner et al., 2018).

Product Class:	Purified
Clone ID:	TP888
Heavy Chain Modification:	His-tagged
Synonyms:	immunoglobulin G1; immunoglobulin G2a; antigen binding fragment; kappa light chain
Amount:	50 μg
Application Codes Clone:	dot blot assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody recognises mouse Fab of the IgG1 and IgG2a which contain kappa light chain (the binding seems to be dependent on the CH1 region and the light chain type simultaneously).
Application Notes:	Anti-IgG nanobodies often perform more efficiently than polyclonal secondary antibodies in various experimental assays. Therefore, they are recommended for various experimental procedures. Anti-IgG nanobodies were shown to be more sensitive during Western blots when conjugated with different molecules (HRP, fluorophores etc.) (Pleiner et al., 2018). Furthermore, combination of two distinct nanobodies for immunofluorescence labelling provided exceptionally clean and strong signal, unprecedented among monovalent secondary antibodies (Pleiner et al., 2018). Additionally, using such an antibody allows skipping two-step incubation procedures during immunostaing as the nanobody can be first incubated with a primary antibody and this conjugate might be added to the cells in a one-step procedure (Pleiner et al., 2018). Finally, such nanobodies have been recommended as very effective for colocalisation studies when more than one fluorophore is needed (Pleiner et al., 2018).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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