Mfg Part Number
Ab01453-1.9
Vector Labs Ab01453-1.9 Anti-IgG2a Fc [TP1129], Mouse IgG1-Fc fusion, His-tagged, (200 μg)
Quick Overview
This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
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| Product Class: | Purified |
|---|---|
| Clone ID: | TP1129 |
| Heavy Chain Modification: | His-tagged |
| Synonyms: | immunoglobulin G2a fragment crystallizable region |
| Amount: | 200 μg |
| Application Codes Clone: | IF; ICC; dot blot assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises Fc region of mouse IgG2a. |
| Application Notes: | TP1129 can be used as an efficient secondary nanobody in the indirect immunofluorescence. It was utilised to detect IgG2a primary antibodies alongside another nanobody giving strong, clean signal without any background staining (Pleiner et al., 2018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |