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Mfg Part Number

Ab01468-10.0

Vector Labs Ab01468-10.0 Anti-CD47 [C5D5], Human IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising Balb/c mice with T84 epithelial membranes.

$517.40

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Description

Product Class: Purified
Clone ID: C5D5
Synonyms: C5/D5; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6
Amount: 200 μg
Application Codes Clone: FC; functional inhibition; IF; IP; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recongises human CD47 extracellular domain. CD47 is a a multitransmembrane glycoprotein widely expressed on all hematopoietic cells and most other cell types. It is involved in cell adhesion, Ca2+ influx, platelet activation and peripheral mononuclear neutrophils migration across endothelial and epithelial monolayers.
Application Notes: C5/D5 is recommended for staining cell surface CD47 as well as neutralising CD47. Furthermore, it is capable of inhibiting peripheral mononuclear neutrophils migration across polarised monolayers of the human intestinal epithelial cell line T84 in a bidirectional fashion (Parkos et al., 1996). This antibody is also very effective in detecting CD47 in flow cytometry; this was demonstrated with various cells, such as CaCO2 and polymorphonuclear neutrophils (Liu et al., 2001). Finally, C5D5 was also utilised in the immunofluorescence staining of the basolateral surfaces of methanol-fixed CaCO2 monolayers expressing exogenously transfected human CD47 (Liu et al., 2001).

Product Class:	Purified
Clone ID:	C5D5
Synonyms:	C5/D5; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6
Amount:	200 μg
Application Codes Clone:	FC; functional inhibition; IF; IP; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recongises human CD47 extracellular domain. CD47 is a a multitransmembrane glycoprotein widely expressed on all hematopoietic cells and most other cell types. It is involved in cell adhesion, Ca2+ influx, platelet activation and peripheral mononuclear neutrophils migration across endothelial and epithelial monolayers.
Application Notes:	C5/D5 is recommended for staining cell surface CD47 as well as neutralising CD47. Furthermore, it is capable of inhibiting peripheral mononuclear neutrophils migration across polarised monolayers of the human intestinal epithelial cell line T84 in a bidirectional fashion (Parkos et al., 1996). This antibody is also very effective in detecting CD47 in flow cytometry; this was demonstrated with various cells, such as CaCO2 and polymorphonuclear neutrophils (Liu et al., 2001). Finally, C5D5 was also utilised in the immunofluorescence staining of the basolateral surfaces of methanol-fixed CaCO2 monolayers expressing exogenously transfected human CD47 (Liu et al., 2001).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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