Vector Labs Ab01470-15.0 Anti-BpaB autotransporter [BpaB#4], Human IgM, kappa (50 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: BpaB#4
Synonyms: Burkholderia mallei BpaB autotransporter; BpaB-4; BpaB 4; BpaB-MAb#4; BpaB MAb#4; BpaB autotransporter of B. mallei
Amount: 50 μg
Application Codes Clone: ELISA; FC; IF; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgGM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for Burkholderia mallei autotransporter BpaB. Autotransporter proteins form one of the largest class of virulence factors in Gram-negative organisms and perform important functions in pathogenesis including flocculation, formation of biofilms , complement resistance, host cell adhesion and entry, intracellular motility and replication, cytotoxicity, and lipolytic activity.
Application Notes: Given the function of autotransporters, such as BpaB in pathogenesis and their overall structure, they are excellent targets for developing medical countermeasures (MCM) against pathogenic organisms. A significant portion of AT (passenger domain) is readily accessible for recognition by the immune system as it is exposed on the bacterial surface. Consequently, BpaB#4 antibody is recommended as a potential research target in treatment and biosafety measures associated with B. mallei. Efficient and specific binding of this antibody to BpaB was confirmed via Western blot; BpaB#4 (BpaB-MAb#4) identified this autotransporter in the material from whole cell lysates (WCL), total membrane proteins (TMP) and sarkosyl-insoluble fraction containing OM proteins (OMP) coming from recombinant E.coli with B. mallei BpaB (Zimmerman et al., 2015). BpaB was shown to be a promising target for the B. mallei therapies due to its involvement in adherence to host cells, biofilm formation and pathogenicity in a mouse model of glanders (Zimmerman et al., 2015). BpaB#4 antibody was further utilised in the identification of B. mallei via immunostaining in various tissues experimentally infected with the bacterium, expanding the antibody's potential use in therapeutics and diagnostics (Zimmerman et al., 2018).

Product Class:	Purified
Clone ID:	BpaB#4
Synonyms:	Burkholderia mallei BpaB autotransporter; BpaB-4; BpaB 4; BpaB-MAb#4; BpaB MAb#4; BpaB autotransporter of B. mallei
Amount:	50 μg
Application Codes Clone:	ELISA; FC; IF; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgGM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for Burkholderia mallei autotransporter BpaB. Autotransporter proteins form one of the largest class of virulence factors in Gram-negative organisms and perform important functions in pathogenesis including flocculation, formation of biofilms , complement resistance, host cell adhesion and entry, intracellular motility and replication, cytotoxicity, and lipolytic activity.
Application Notes:	Given the function of autotransporters, such as BpaB in pathogenesis and their overall structure, they are excellent targets for developing medical countermeasures (MCM) against pathogenic organisms. A significant portion of AT (passenger domain) is readily accessible for recognition by the immune system as it is exposed on the bacterial surface. Consequently, BpaB#4 antibody is recommended as a potential research target in treatment and biosafety measures associated with B. mallei. Efficient and specific binding of this antibody to BpaB was confirmed via Western blot; BpaB#4 (BpaB-MAb#4) identified this autotransporter in the material from whole cell lysates (WCL), total membrane proteins (TMP) and sarkosyl-insoluble fraction containing OM proteins (OMP) coming from recombinant E.coli with B. mallei BpaB (Zimmerman et al., 2015). BpaB was shown to be a promising target for the B. mallei therapies due to its involvement in adherence to host cells, biofilm formation and pathogenicity in a mouse model of glanders (Zimmerman et al., 2015). BpaB#4 antibody was further utilised in the identification of B. mallei via immunostaining in various tissues experimentally infected with the bacterium, expanding the antibody's potential use in therapeutics and diagnostics (Zimmerman et al., 2018).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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