Mfg Part Number
Ab01471-15.0

Vector Labs Ab01471-15.0 Anti-Yersinia pestis surface antigens [6B5], Human IgM, kappa (50 μg)

Quick Overview
This antibody was raised by immunising Balb/c mice with formalin-killed F1 antigen–deficient Yersinia pestis A1122 cells.
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Product Class:Purified
Clone ID:6B5
Synonyms:non-fraction-1 cell membrane; Yersina pestis surface antigen; Y. pestis non-fraction-one cell membrane antigen; F1 deficient Yersinia pestis; Yersinia pestis F1 deficient antigen; fraction 1 antigen; Yersinia pestis; Pasteurella pestis; Bacterium pestis; Bacille de la peste
Amount:50 μg
Application Codes Clone:ELISA; WB
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody recognises Y. pestis surface antigen other than F1 (Western blot analysis showed that this antigen is an outer membrane protein of ca. 30 kDa). Y. pestis It is a facultative anaerobe that can infect humans causing plague. 6B5 antibody also exhibits minor cross-reactivity with Y. pseudotuberculosis. It does not cross-react with the following species: Y. enterocolitica, Salmonella Typhimurium, Salmonella Enteritidis, Salmonella Cerro, Salmonella Montevideo, Salmonella Medegridis, C. freundii, L. monocytogenes, L. innocua, E. coli, E. coli O157:H7, E. coli O111:NM, E. coli O26:H11, S. marcescens, V. cholerae, K. pneumoniae, K. oxytoca, A. viridans, H. alvei, S. flexneri, S. sonnei, P. hauseri.
Application Notes:This antibody allows detection of Yersinia pestis in room temperature. Most available immunosassays for Y. pestis are based on detection of F1, the dominant surface antigen expressed by the pathogen when it is grown at 37C. The advantage of detecting Y. pestis at room temperature is that it allows contamination testing in water, juice, milk, food and other environmental samples (Zhao et al., 2012). This antibody is recommended for the detection of Yersinia pestis in various samples as well as for the research and construction of Y. pestis tests and diagnostic tools.
More Information
Brand Vector Labs
UNSPSC 12161500