Mfg Part Number
Ab01472-15.0
Vector Labs Ab01472-15.0 Anti-Matrix Protein [E10], Human IgM, kappa (50 μg)
Quick Overview
This antibody was raised by immunising mice with the matrix protein 2 (M2) extracellular domain peptide-KLH conjugate.
$692.90
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| Product Class: | Purified |
|---|---|
| Clone ID: | E10 |
| Synonyms: | M; Matrix protein 1; Matrix protein 2; M1; M2; Proton channel protein M2; M2-E10 |
| Amount: | 50 μg |
| Application Codes Clone: | WB; ELISA; IHC; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises both M1 and M2 proteins of all Influenza A viruses. The M1 protein is critical for assembly and budding, interacting with the influenza hemagglutinin (HA) and neuraminidase (NA) proteins. M2 is a transmembrane protein that has been found to play a role in Influenza replication and assembly of virion particles. It has been shown that this protein is an acid-activated ion channel for virus replication. |
| Application Notes: | E10 antibody may be used both to detect Influenza A viruses and to identify proteins M1 and M2. THis antibody was used to copare the amount of M1 and M2 proteins between wild-type and recombinant Influenza viruses (Bourmakina and García-Sastre, 2005). It was also utilised in the flow cytometric analysis of the knockdown efficiency of M2 protein (Gannagé et al., 2009). Finally, E10 antibody successfully immunostained MDCK cells in experiments attempting at establishing the titers of the virus (Ramos et al., 2013). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |