Mfg Part Number
Ab01489-2.0
Vector Labs Ab01489-2.0 Anti-CD19 [6D5], Mouse IgG2a, kappa (200 μg)
Quick Overview
This antibody was raised by immunising rats with mouse CD19-expressing K562 human erythroleukemia cells.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 6D5 |
| Synonyms: | Leu12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19 T-cell surface antigen Leu-12; CD19; B4 |
| Amount: | 200 μg |
| Application Codes Clone: | FC; IHC; IP; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises mouse CD19, which is a transmembrane glycoprotein that regulates B lymphocyte development, activation, and differentiation. This clone 6D5 recognizes the same epitope as the published clone 1D3. |
| Application Notes: | 6D5 antibody has been extensively used to identify B cells in various experimental arrangements. It can be efficiently utilised in the localisation of B cells in various tissues. For instance, one group stained murine spleen tissue cryosections to visualise lymphomas in azathioprine-treated mice (Chalastanis et al., 2010). This antibody has also been widely used in flow cytometry. One group utilised in the flow cytometric identification of B cells in a study investigating the relation of microglia depletion and MPTP neurotoxicity (Yang et al., 2018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |