Mfg Part Number
Ab01491-1.6-BS

Vector Labs Ab01491-1.6-BS Anti-CD115/M-CSFR [AFS98], Mouse Fab fragment, His-Tagged, kappa (1 mg)

Quick Overview
This antibody was raised against CD115.
$5,162.30
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Product Class:Purified
Clone ID:AFS98
Heavy Chain Modification:His-Tagged
Synonyms:CSF-1R; Macrophage colony-stimulating factor 1 receptor; Csf1r; CD115; CSF-1 receptor; EC 2.7.10.1; CSF-1-R; M-CSF-R; Proto-oncogene c-Fms
Amount:1 mg
Application Codes Clone:FC; blocking; WB; IHC
Shipping Temperature:Wet Ice
Buffer Composition:PBS only.
Original Format:IgG2a
Storage Temperature:Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody recognises mouse M-CSFR (CD115, CSF-1R) which is a membrane protein that influences tissue macrophage and osteoclast differentiation and proliferation.
Application Notes:AFS98 has been widely used in various flow cytometric analyses to label CD115 antigen. For instance, one group used it to detect monocytes in a mouse blood sample (Buschor et al., 2019). Similarly, Huo and colleagues (2017) utilised this antibody to identify monocytes in mouse blood in order to assess whether myelopoiesis was not a cause of the increase in the monocytes number. This antibody has been also successfully used to deplete macrophages (Ho et al., 2016). Finally, it was also utilised in the immunofluorescence staining of tissue sections prepared from 4% paraformaldehyde-fixed and decalcified bones (Dong et al., 2016).
More Information
Brand Vector Labs
UNSPSC 12161500