Mfg Part Number
Ab01532-1.1
Vector Labs Ab01532-1.1 Anti-ssRNA (sequence specific) [BRG (5E08)], Mouse IgG1, kappa (200 μg)
Quick Overview
This Fab antibody fragment was raised using YSGR phage display library against a branched RNA fragment corresponding in sequence to the branch region of the pre-mRNA intron derived from the YBL059W gene from S. cerevisiae.
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| Product Class: | Purified |
|---|---|
| Clone ID: | BRG (5E08) |
| Synonyms: | single stranded RNA; RNA; ribonucleic acid |
| Amount: | 200 μg |
| Application Codes Clone: | structural analysis; functional assays |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds to an RNA fragment of a minimal 10 nt sequence N3U4G5C6A7N8A9G10G11N12. |
| Application Notes: | It was demonstrated that the binding of BRP Fab fragment to the RNA molecule stabilised an RNA hairpin structure which was not steady in the absence of the Fab (Shao et al., 2016). Consequently, BRP might be used to capture dynamic RNAs in distinct conformational states and allow structural analysis of nucleic acids. Additionally, BRG Fab antibody fragment was shown to inhibit Dbr1 debranching of the RNA target (Shao et al., 2016). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |