Mfg Part Number
Ab01533-8.4
Vector Labs Ab01533-8.4 Anti-pectinase [B1], Rat IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
Spleen mRNA from mice immunized with extracellular proteins from Rhizoctonia solani was used to construct a cDNA library from which antipectinase ScFv antibodies were isolated using phage display techniques (Manatunga et al., 2005).
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| Product Class: | Purified |
|---|---|
| Clone ID: | B1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Polygalacturonase; PG; Endopolygalacturonase; ScFvB1; AY741402; enzyme pectinase |
| Amount: | 200 μg |
| Application Codes Clone: | WB; ELISA; functional assays |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rat antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds to pectinases from various pathogenic fungi from such groups as ascomycetes, basidiomycetes, and oomycete. |
| Application Notes: | B1 scFV antibody was shown to inhibit polygalacturonase (PG) activity in a number of pathogenic species of fungi and thus, it is a highly promising agent in the research on the methods of plant fungal diseases prevention (Manatunga et al., 2005). Furthermore, this antibody was also able to inhibit maceration caused by infection of plant tissue by necrotrophic fungi (Manatunga et al., 2005). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |