Mfg Part Number
Ab01541-3.0

Vector Labs Ab01541-3.0 Anti-CD43 [84-3C1], Mouse IgG2b, kappa (200 μg)

Quick Overview
This antibody was raised by immunizng mice with stimulated human leukocytes.
$517.40
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Product Class:Purified
Clone ID:84-3C1
Synonyms:Leukosialin; Sialophorin; GPL115; Galactoglycoprotein; GALGP; Leukocyte sialoglycoprotein
Amount:200 μg
Application Codes Clone:WB; FC; IP; IF; IHC
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2b
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:This antibody recognizes CD43 which is a predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration.
Application Notes:This antibody can be used in the identification of malignant lymphomas. CD43 is expressed on 22-37% of B-cell lymphomas and, at the same time, it is not expressed on reactive B-cells. Consequently, B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma. If used with an anti-CD20 antibody, this clone may be utilized in the immunophenotyping of the lymphomas in formalin-fixed tissues, as Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 usually indicates lymphoma. Importantly, 84-3C1 antibody can be used for the detection of CD43 via various methods. For instance, it was successful in indetifying that antigen in a lysate of dendritic cells' platellet via Western blot (Julien et al., 2007). Finally, another group used this antibody during flow cytometric analysis a sample of B-cells (Yoon et al., 2017).
More Information
Brand Vector Labs
UNSPSC 12161500