Mfg Part Number
Ab01542-10.3
Vector Labs Ab01542-10.3 Anti-CD53 [161-2 (53/2)], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunizng mice with stimulated human leukocytes.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 161-2 (53/2) |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Tetraspanin-25; Leukocyte surface antigen CD53; Cell surface glycoprotein CD53; Tspan-25 |
| Amount: | 200 μg |
| Application Codes Clone: | FC; IF; functional studies |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes human CD53. It does not react with the baboon and horse antigen. Cd53 is a member of tetraspanin family and is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. |
| Application Notes: | This antibody is recommended for the detection and analysis of CD53 via flow cytometry and immunofluorescence. Additionally, one group utilized this antibody for the ligation of the CD53 antigen while researching the potential involvement of tetraspanins (such as CD53) in tumor metastasis (Yunta et al., 2002). Furthermore, CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells, as it is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |