Mfg Part Number
Ab01544-23.0
Vector Labs Ab01544-23.0 Anti-CD63 [NK-1-C3], Rabbit IgG, kappa (200 μg)
Quick Overview
This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | NK-1-C3 |
| Synonyms: | Granulophysin; LAMP-3; Tetraspanin-30; Tspan-30; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; CD63 antigen; NK1/C3; NKI/C3; NK-1.C3; NK1C3 |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IP; IF; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes CD63. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation and plays a role in the activation of cellular signaling cascades. |
| Application Notes: | The antibody NK-1-C3 has been widely used for the detection and analysis of CD63 via various methods. For instance, NK-1-C3 (NK1/C3) proved successfull in labelling CD63 in multiple tissues, for example, when localizing CD63 in murine retinal pigment epithelium (RPE)/choroid tissue (Wang et al., 2009). Similarly, this antibody was used to detect CD63 on melanoma cells via immunofluorescence staining (Khazen et al., 2016). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |