Mfg Part Number
Ab01557-2.3
Vector Labs Ab01557-2.3 Anti-Complement C3 [1H8], Mouse IgG2a, Fc Silent™, kappa (200 μg)
Quick Overview
The mouse version of this antibody was raised by immunizing a mouse with human C3b and iC3b bound to a carbohydrate matrix via the alternative pathway of complement activation.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1H8 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Complement C3; C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IF; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for human C3b. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. |
| Application Notes: | To show that a monoclonal antibody directed against the merozoite and human polyclonal IgG from merozoite vaccine recipients enhanced RBC invasion in a complement-dependent manner, immunofluorescence was performed on merozoites with RBC using the mouse version of this antibody (Biryukov et al, 2016; pmid:27333049). Flow cytometry was performed on intact and lysed PNH erythrocytes using the mouse version of this antibody to analyze the deposition of C3 (Lindorfer et al, 2010; pmid:20068220). While studying the role of the complement system in childhood malaria, immunoprecipitation was performed on erythrocytes using the mouse version of this antibody. Furthermore, a western blot was performed on erythrocytes using this antibody (Pawluczkowycz et al, 2007; pmid:17911641). To examine the ability of several commercially available phycoerythrin (PE)-labeled anti-Mouse IgG antibodies to bind to B cells opsonized in milieu which allow or preclude complement activation, flow cytometry was performed on Raji cells, Z138C cells or CLL patient B cells using the mouse version of this antibody. Furthermore, Raji cells, Z138C cells or CLL patient B cells were stained for immunofluorescence using this antibody (Beum et al, 2004; pmid:15604014). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |