Mfg Part Number
Ab01570-2.0
Vector Labs Ab01570-2.0 Anti-Triplex DNA [Jel 466], Mouse IgG2a, kappa (200 μg)
Quick Overview
Jel 466 was prepared by immunizing mice with poly[d(Tm5C)].poly[d(GA)].
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| Product Class: | Purified |
|---|---|
| Clone ID: | Jel 466 |
| Synonyms: | triple helix DNA; Jel-466; Jel466 |
| Amount: | 200 μg |
| Application Codes Clone: | IF |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 7509814 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | Jel 466 binds to triple-stranded DNA. The preferred interacting partner is the triplex form of poly[d(TC)]. poly[d(GA)] and there is also good binding to poly[d(TTC)].poly[d(GAA)]. Theantibody binds weakly to the triplex derived from poly[d(G)].poly[d(C)] and no binding is observed with poly[d(T)].poly[d(A)1.poly[d(T)]. Jel 466 does not bind to poly[d(TC)].poly[d(Gm6A)], single-stranded DNAs, calf thymus DNA, or to poly[d(TG)].poly[d(CA)] which are unable to form a triplex structure. Triplex DNA has been known to form in vitro from synthetic pyrimidine-pruine tracts and it is also thought to potentially have a biological function in cells. These functions include the control of gene expression due to the presence of pyrimidine-purine tracts in the 5' control region and chromosomal organization whereby DNA loops can be stabilised by triplexes (some MARs [matrix attched regions] contain triplez-forming sequences). |
| Application Notes: | Jel 466 can be used to detect triplex DNA in cells by IF. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |