Mfg Part Number
Ab01572-10.3
Vector Labs Ab01572-10.3 Anti-OspA [184.1], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
BALB/c mice were immunised by injection of B. burgdorferi sonicated preparations. Splenocytes were obtained from immunised mice and fused with X63-Ag8 myeloma cells to generate hybridomas.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 184.1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Outer surface protein A |
| Amount: | 100 μg |
| Application Codes Clone: | WB; ELISA; crystallisation |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 9108020 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | 184.1 was shown to react specifically with a common OspA epitope shared by a homologous protein of lower molecular weight (Jiang et al, 1990). Based on N-terminal sequencing and reactivity of 184.1 against truncated versions of OspA, it was concluded that 184.1 recognises an N-terminal epitope of OspA. This was corroborated by a crystal structure of the OspA-184.1 Fab structure (Li et al, 1997), which showed that 184.1 binds a well-ordered beta sheet arrangement near the N-terminus. |
| Application Notes: | 184.1 was used in Western blots and ELISA to determine specificity against B. burgdorferi major surface proteins (Jiang et al, 1990). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |