Mfg Part Number
Ab01573-22.0
Vector Labs Ab01573-22.0 Anti-CD79b [HM79-16], Hamster (Armenian) IgG, lambda (200 μg)
Quick Overview
HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).
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| Product Class: | Purified |
|---|---|
| Clone ID: | HM79-16 |
| Synonyms: | Ig-beta; AGM6; B29; IGB; CD79b molecule; |
| Amount: | 200 μg |
| Application Codes Clone: | FC; IP; IF; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | Reacts with an extracellular epitope of CD79b. |
| Application Notes: | A biotinylated HM79-16 was used and stained with streptavidin-FITC for analysis by FACS to identify variants of B lymphoma cell lines that have lost expression of the mu heavy chain, and for staining using a biotinylated HM79-16 (Condon 2000). HM79-16 has also been used in competitive binding assays to study its effect on the binding of CD79a to CD79b in the context of ameliorating autoimmune disease in mice (Yongmei Li 2008). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |