Mfg Part Number
Ab01577-10.3

Vector Labs Ab01577-10.3 Anti-CCR5 [T21/8], Human IgG1, Fc Silent™, kappa (200 μg)

Quick Overview
This antibody was raised by immunizing mice with intact RBL-CCR5 cells.
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Product Class:Purified
Clone ID:T21/8
Heavy Chain Modification:Fc Silent™
Synonyms:CD195; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor
Amount:200 μg
Application Codes Clone:WB; IF; FC; ELISA; IP
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2a
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:T21/8 antibody binds with high affinity to a CCR5 N-terminal epitope. CCR5 is a receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES.
Application Notes:T21/8 antibody is recommedned for the detection and analysis of CCR5 via various methods. For instance, one group used this antibody to detect intracellular localization of CCR5 in RBL-CCR5 cells via immunofluorescence staining (Pollok-Kopp et al., 2003). Another group investigated ligand-mediated down-modulation of CCR5 via flow cytometry, utilizing T21/8 antibody (Fox et al., 2015). With other anti-CCR5 antibodies which react with CCR5 phosphorylated on different serine residues (Ab01578; Ab01579), it can be used to study dynamic regulation of CCR5 and phosphorylation mechanisms.
More Information
Brand Vector Labs
UNSPSC 12161500