JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab01617-23.0

Vector Labs Ab01617-23.0 Anti-CD40 [2C5], Rabbit IgG, kappa (200 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

This antibody was raised by immunising mice with a recombinant protein corresponding to the extracellular domain of chicken CD40 (chCD40ED).

$517.40

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: 2C5
Synonyms: CD40L receptor; CDw40; Bp50; B-cell surface antigen CD40; Tumor necrosis factor receptor superfamily member 5
Amount: 200 μg
Application Codes Clone: agonist; immune activation; FC; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognises extracellular domain of chicken CD40. CD40, an integral membrane glycoprotein of the TNF-receptor superfamily, is expressed mainly on professional antigen-presenting cells (APCs), including B-cells, macrophages, and dendritic cells. However, high levels of CD40 can also be detected on a wide range of tumor cells of the bladder, breast, and ovary.
Application Notes: 2C5 antibody is a CD40 agonist and can be used to induce immune activation. Agonistic monoclonal anti-CD40 antibodies (mAbs) mimic the effects of CD40L on APCs, leading to enhanced T-cell priming andexpansion, increased antibody production and class switching. This antibody was shown to be a powerful adjuvant eliciting a strong IgG-response in chicken what makes it an important agent in vaccines development (Chen et al., 2012). Furthermore, 2C5 was also demonstrated to be effective in detecting CD40 via immunofluorescence as well as via flow cytometry in chicken B cells and macrophages (Chen et al., 2010). Finally, this antibody was observed to activate NO synthesis in HD11 macrophages, and stimulate DT40 B-cellproliferation (Chent et al., 2010).

Product Class:	Purified
Clone ID:	2C5
Synonyms:	CD40L receptor; CDw40; Bp50; B-cell surface antigen CD40; Tumor necrosis factor receptor superfamily member 5
Amount:	200 μg
Application Codes Clone:	agonist; immune activation; FC; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognises extracellular domain of chicken CD40. CD40, an integral membrane glycoprotein of the TNF-receptor superfamily, is expressed mainly on professional antigen-presenting cells (APCs), including B-cells, macrophages, and dendritic cells. However, high levels of CD40 can also be detected on a wide range of tumor cells of the bladder, breast, and ovary.
Application Notes:	2C5 antibody is a CD40 agonist and can be used to induce immune activation. Agonistic monoclonal anti-CD40 antibodies (mAbs) mimic the effects of CD40L on APCs, leading to enhanced T-cell priming andexpansion, increased antibody production and class switching. This antibody was shown to be a powerful adjuvant eliciting a strong IgG-response in chicken what makes it an important agent in vaccines development (Chen et al., 2012). Furthermore, 2C5 was also demonstrated to be effective in detecting CD40 via immunofluorescence as well as via flow cytometry in chicken B cells and macrophages (Chen et al., 2010). Finally, this antibody was observed to activate NO synthesis in HD11 macrophages, and stimulate DT40 B-cellproliferation (Chent et al., 2010).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews