Mfg Part Number
Ab01640-23.9-CBT

Vector Labs Ab01640-23.9-CBT Anti-Vimentin [1433], Rabbit IgG-Fc fusion, His-Tagged, (1 mg)

Quick Overview
The original antibody was generated from an immunized alpaca.
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Product Class:Purified formulation
Clone ID:1433
Heavy Chain Modification:His-Tagged
Synonyms:VIM
Amount:1 mg
Application Codes Clone:IF
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 10% glycerol
Original Format:VHH
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This is a chimeric rabbit antibody, based on the original camelid VHH format, created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody binds the vimentin intermediate filament. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Application Notes:This chimeric engineered antibody is generated by fusing the original alpaca derived nanobody to mouse IgG1 and rabbit Fc domains to extend the research application of the original. This antibody binds vimentin present on canine MDCK cells, rodent BHK cells and on human A549 cells upon stimulation with TGFβ. This antibody can be used for immunofluorescent staining of tissues expressing vimentin. It can also be conjugated with fluorophores for direct detection. It can also be immobilized on protein A, beads, resins and plates. The original alpaca VHH antibody is available from ChromoTek.
More Information
Brand Vector Labs
UNSPSC 12161500