Mfg Part Number
Ab01642-30.11
Vector Labs Ab01642-30.11 Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7], scFv fragment (His), ScFv, (50 μg)
Quick Overview
The antibody was raised using IONTAS phage display library, a naïve human scFv library consisting of 4.0 · 10^10 unique antibody fragments. Through phage display selection, scFv-presenting phages were allowed to bind a toxin, phages with unspecific binding were washed away, and the rest were eluted through enzymatic digestion. These were then amplified and used for the following round. In total three rounds of selections were completed leaving the best binders behind.
$692.90
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | TPL0027_01_F7 |
| Heavy Chain Modification: | ScFv |
| Synonyms: | CTX1,3,7; CX1; Cardiotoxin F8; Cardiotoxin-I; CTX-I; Toxin CM-6; Cytotoxin 3; CX3; Toxin CM-7; Cytotoxin 7; Toxin CM-4B |
| Amount: | 50 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | TPL0027_01_F7 antibody recognizes cytotoxins 1, 3 and 7, which are some of the major toxins in the venom of the black-necked spitting cobra (Naja nigricollis). |
| Application Notes: | TPL0027_01_F7 antibody has high affinity to the cytotoxins 1, 3 and 7. It was demonstrated by normalized ELISA, which circumvents the limitation of a simple ELISA by removing the expression variable, normalizing the amount of scFv in each well. The antibody's high affinity was confirmed by its ability to bind the target proteins in a venom fraction even at 1:32,000 dilution (3.125 · 10^-2) µg/mL (Jürgensen et al., 2019 [poster]). It is recommended for the research on the antitoxin against Naja nigricollis snakebites. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |