Mfg Part Number
Ab01649-1.1
Vector Labs Ab01649-1.1 Anti-CaMPARI-Red [4F6], Mouse IgG1, kappa (200 μg)
Quick Overview
This antibody was originally raised by immunizing mice with a purified and photoconverted EosFP protein, subjected it to proteolysis, and further purified the proteolytic fragment containing the red chromophore which is identical to the equivalent region of CaMPARI1 and CaMPARI2.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4F6 |
| Synonyms: | CaMPARI 1&2; CaMPARI1; CaMPARI2; CaMPARI-Red; Calcium Modulated Photoactivatable Ratiometric Integrator; 4F6-1 |
| Amount: | 200 μg |
| Application Codes Clone: | IHC; WB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for the photoconverted form of CaMPARI-Red (both CaMPARI1 and CaMPARI2). CaMPARI is s a photoconvertible protein construct that allows imaging of the integrated calcium activity of populations of cells over time. |
| Application Notes: | 4F6 is recommended for the detection and analysis of CaMPARI via immunohistochemistry (IHC) and Western blot (WB) (Moeyaert et al., 2018; PMID: 30361563). For instance, it was successfully used in the immunohistochemical identification of CaMPARI-Red cells in brain tissue, distinguishing them from the cells in which CaMPARI stayed in ints green, unconverted form (Ebner et al., 2019; PMID: 31178713). This product (Ab01649-23.0) was used for indirect immunofluorescence (IF) staining of adult female Drosophila fly brains to visualize and detect the localization of the CaMPARI-Red protein (Gendron et al., 2023; PMID: 37310911). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |