Vector Labs Ab01651-23.0 Anti-CD40 [2E4E4], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 2E4E4
Synonyms: CD40L receptor; CDw40; Bp50; B-cell surface antigen CD40; Tumor necrosis factor receptor superfamily member 5
Amount: 200 μg
Application Codes Clone: agonist; immune activation; IHC; ICC; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognises extracellular domain of swine, bovine, caprine, and ovine CD40. CD40, an integral membrane glycoprotein of the TNF-receptor superfamily, is expressed mainly on professional antigen-presenting cells (APCs), including B-cells, macrophages, and dendritic cells. However, high levels of CD40 can also be detected on a wide range of tumor cells of the bladder, breast, and ovary.
Application Notes: 2E4E4 antibody is recommended as an effective adjuvant in the development of vaccines as it was demonstrated to have strong agonistic activity against CD40 evoking 25-fold or greater proliferation of peripheral blood mononuclear cells (PBMCs) from livestock species relative to cells incubated with an isotype control (Martin et al., 2017; PMID: 28107431). What is more, it led to a strong nitric oxide release from bovine macrophages, enhanced expression of MHC-II by PBMCs and induced significant expression of IL-1α, IL6, IL-8, and TNF-α by PBMCs (Martin et al., 2017; PMID: 28107431). Furthermore, this antibody was successfully utilised in the detection of bovine, caprine, ovine and swine CD40 via flow cytometry and immunohistochemistry in the spleen tissue samples (Martin et al., 2017; PMID: 28107431).

Product Class:	Purified
Clone ID:	2E4E4
Synonyms:	CD40L receptor; CDw40; Bp50; B-cell surface antigen CD40; Tumor necrosis factor receptor superfamily member 5
Amount:	200 μg
Application Codes Clone:	agonist; immune activation; IHC; ICC; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognises extracellular domain of swine, bovine, caprine, and ovine CD40. CD40, an integral membrane glycoprotein of the TNF-receptor superfamily, is expressed mainly on professional antigen-presenting cells (APCs), including B-cells, macrophages, and dendritic cells. However, high levels of CD40 can also be detected on a wide range of tumor cells of the bladder, breast, and ovary.
Application Notes:	2E4E4 antibody is recommended as an effective adjuvant in the development of vaccines as it was demonstrated to have strong agonistic activity against CD40 evoking 25-fold or greater proliferation of peripheral blood mononuclear cells (PBMCs) from livestock species relative to cells incubated with an isotype control (Martin et al., 2017; PMID: 28107431). What is more, it led to a strong nitric oxide release from bovine macrophages, enhanced expression of MHC-II by PBMCs and induced significant expression of IL-1α, IL6, IL-8, and TNF-α by PBMCs (Martin et al., 2017; PMID: 28107431). Furthermore, this antibody was successfully utilised in the detection of bovine, caprine, ovine and swine CD40 via flow cytometry and immunohistochemistry in the spleen tissue samples (Martin et al., 2017; PMID: 28107431).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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