Mfg Part Number
Ab01654-10.3
Vector Labs Ab01654-10.3 Anti-alpha catenin [1G5], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunizing Balb/c mice with a 90 kDa MBP/alpha catenin fusion protein purified on an amylose column.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1G5 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Catenin alpha-1; Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13; α-catenin; α catenin; α(E)-catenin |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IF; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes alpha catenin and binds to the domain between amino acids 135-226. α-catenin associates with the cytoplasmic domain of a variety of plasma membrane spanning cadherins that mediate cell-cell adhesion. The association of catenins to cadherins produces a complex that is linked to the actin filament network, which seems to be of primary importance for their cell-adhesion properties. |
| Application Notes: | This antibody is recommended for the detection and analysis of alpha-catenin via various methods. For instance, it was used to identify alpha-catenin in MDA468 cells via immunofluorescence (Bajpai et al., 2009). It was also repeatedly demonstrated that 1G5 specifically detects alpha-catenin on Western blots (Johnson et al., 1993; Stadler et al., 2018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |