Mfg Part Number
Ab01665-3.0
Vector Labs Ab01665-3.0 Anti-SARS-CoV S glycoprotein [1B5], Mouse IgG2b, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing a non human transgenic animal XENOMOUSE® IgG2k mice against S1 protein-Ig fragments (ectodomain) of the S Protein (Tor2).
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1B5 |
| Synonyms: | Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1 |
| Amount: | 200 μg |
| Application Codes Clone: | NTRL; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG2 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody specifically binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein (Urbani strain). |
| Application Notes: | This antibody inhibits the association of SARS-CoV s protein to the angiotensin-converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the virus. The originally characterized antibody had a neutralizing titer 200TCID50 of 12.5 μg/ml (Coughlin et at.,2006) and IC50 of 2.84 μg/ml when incubated with S12–510- Fc recombinant protein (Coughlin et at.,2009). Purified 1B5 binds to RBD region with a unique CDR3 sequence. When used in combination with 4D4 the lack of synergistic effect indicated that the mAbs 4D4 and 1B5 likely interact with closely positioned conformational epitopes and compete with each other for binding (Coughlin et at.,2009). ELISA. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |