Vector Labs Ab01666-21.0 Anti-SARS-CoV S glycoprotein [4D4], Mouse IgM, kappa (50 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 4D4
Synonyms: Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1
Amount: 50 μg
Application Codes Clone: NTRL; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG2 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody specifically binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein (Urbani strain).
Application Notes: This antibody inhibits the association of SARS-CoV s protein to the angiotensin-converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the virus. The originally characterized antibody had a neutralizing titer 200TCID50 of 12.5 μg/ml (Coughlin et at.,2006). When incubated with S12–510- Fc recombinant protein it failed to block the interaction of this protein with the cellular receptor but inhibited a post-binding event and prevented viral entry. Combination of this antibody with another antibody 5D3, that binds to a different epitope resulted in increased inhibition of virus. The ability of mAb 4D4 to more efficiently inhibit viral entry when added post virus binding suggests that the S protein might undergo a conformational change following binding to ACE2 leading to unmasking of the epitope recognized by the 4D4 antibody. Simultaneously, 4D4 was also used in a standardentry inhibition assay as a positive control for determining the degree of inhibition of pseudotyped virus entry. (Coughlin et at.,2009). This antibody 4D4 showed about 50% binding to Sin845 S1 protein relative to their binding to Urbani S1 protein. GZ0402-S1 protein showed minimal of 57% binding to HmAbs 4D4 (Elshabrawy et al., 2012). Other applications include ELISA.

Product Class:	Purified
Clone ID:	4D4
Synonyms:	Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1
Amount:	50 μg
Application Codes Clone:	NTRL; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG2 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody specifically binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein (Urbani strain).
Application Notes:	This antibody inhibits the association of SARS-CoV s protein to the angiotensin-converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the virus. The originally characterized antibody had a neutralizing titer 200TCID50 of 12.5 μg/ml (Coughlin et at.,2006). When incubated with S12–510- Fc recombinant protein it failed to block the interaction of this protein with the cellular receptor but inhibited a post-binding event and prevented viral entry. Combination of this antibody with another antibody 5D3, that binds to a different epitope resulted in increased inhibition of virus. The ability of mAb 4D4 to more efficiently inhibit viral entry when added post virus binding suggests that the S protein might undergo a conformational change following binding to ACE2 leading to unmasking of the epitope recognized by the 4D4 antibody. Simultaneously, 4D4 was also used in a standardentry inhibition assay as a positive control for determining the degree of inhibition of pseudotyped virus entry. (Coughlin et at.,2009). This antibody 4D4 showed about 50% binding to Sin845 S1 protein relative to their binding to Urbani S1 protein. GZ0402-S1 protein showed minimal of 57% binding to HmAbs 4D4 (Elshabrawy et al., 2012). Other applications include ELISA.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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