Mfg Part Number
Ab01669-10.0
Vector Labs Ab01669-10.0 Anti-SARS-CoV S glycoprotein [A11], Human IgG1, lambda (200 μg)
Quick Overview
The original antigen was produced by subcloning the scFv gene fragment in an expression vector like pET32b, and transforming the vector into an E.coli strain BL21 and inducing expression by IPTG ( isopropyl-b-D-thiogalactopyranoside). A scFv phage antibody library was constructed in fd phage. The antibody was produced by panning against S2b, two non-immune human Ab phage display libraries and neutralizing activity was tested against pseudotyped viruses. Antigen binding antibodies were identified by phage ELISA.
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| Product Class: | Purified |
|---|---|
| Clone ID: | A11 |
| Synonyms: | Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S2 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IF; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody specifically binds the epitope in the S2 domain of the spike protein in the region of Leu803-Ala828. |
| Application Notes: | This antibody binds the amino acids Leu803-Ala828 in the S2 domain of the SARS-CoV. S2 domain mediates membrane fusion between the virus and the host cell. The binding specificity of antibody to S2b fragment of the viruse was checked by ELISA. Immunofluorescence staining was used to stain infected Vero cells using the A11-scFv. A11-scFv recognized recombinant spike protein S2-b during western blot analysis (Leung et al., 2008). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |