Mfg Part Number
Ab01693-10.3
Vector Labs Ab01693-10.3 Anti-Plakophilin 1 [10B2], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunizing mice with the N-terminus of human Plakophilin-1 protein.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 10B2 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | PKP1; PKP-1; Plakophilin-1; Band 6 protein; B6P; PKP 1 |
| Amount: | 200 μg |
| Application Codes Clone: | FC; IF; WB; IP; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes Plakophilin-1. It binds the epitope within the first 27 amino acids from the N-terminal region and it does not cross react with Plakophilin 2 and Plakophilin 3. Plakophilins influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilin-1 is thought to play a role in junctional plaques and contribute to epidermal morphogenesis. |
| Application Notes: | 10B2 is recommedned for the detection and analysis of Plakophilin-1. In order to identify potential perturbations in cell adherence, this antibody was used to detect plakophilin-1 in the skin tissue of patients with epidermolysis bullosa via immunofluorescence (Pigors et al., 2011; pmid: 21320868). Further, this antibody was demonstrated to have a potential diagnostic role in certain diseases, such as Ectodermal Dysplasia/Skin Fragility Syndrome where it was used to differentiate between a diseased and healthy skin samples (Sprecher et al., 2004; pmid: 15086548). It was also shown that 10B2 antibody can detect plakophilin-1 (PKP1) via Western blot (here: in HaCaT protein lysates) (Bonne et al., 2003; pmid: 12707304). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |